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Published In
Archives of Rheumatology
Volume 37 · Issue 4 · December 2022
Pages: 536-546
History
Published Online: December 31, 2022
This work is licensed under a Creative Commons Attribution 4.0 International License.
Fang Li
Department of Rheumatism and Immunology, Hebei General Hospital, Shijiazhuang, China
Jian-Hua Yao
Department of Geratology, The Second Hospital of Hebei Medical University, Shijiazhuang, China
Li Li
Department Ultrasonograph, Hebei General Hospital, Shijiazhuang, China
Qian Nie
Hebei General Hospital, Medicai Examination Center, Shijiazhuang, China
Jing-Jing Cao
Department of Rheumatism and Immunology, Hebei General Hospital, Shijiazhuang, China
Xiao-Ran Ning
Department of Rheumatism and Immunology, Hebei General Hospital, Shijiazhuang, China
Fang Li, Jian-Hua Yao, Li Li, Qian Nie, Jing-Jing Cao, & Xiao-Ran Ning. (2022). MiRNA-23a-5p is the biomarkers for gouty arthritis and promotes inflammation in rats of gouty arthritis via MyD88/NF-κB pathway by induction TLR2. Archives of Rheumatology, 37(4), 536–546. https://doi.org/10.46497/ArchRheumatol.2022.9236
Abstract
Objectives: In this study, we aimed to examine the efficacy of micro ribonucleic acid (miRNA)-23a-5p in gouty arthritis and to investigate its possible mechanism.
Materials and methods: Gouty arthritis in rat was established by intraarticular injection of 0.2 mL monosodium urate crystal (20 mg/mL) inside knee joint cavity. THP-1 cell was induced using lipopolysaccharides (LPS) for in vitro model.
Results: Serum miRNA-23a-5p expression levels were increased in rats of gouty arthritis. However, overexpression of miRNA-23a-5p promoted inflammation and induced myeloid differential protein-88 (MyD88)/nuclear factor-kappa B (NF-κB) pathway by induction toll-like receptor-2 (TLR2) in vitro. The inhibition of TLR2 attenuated the pro-inflammation effects of miRNA-23a-5p in inflammation in in vitro model of gouty arthritis.
Conclusion: Our findings demonstrate that miRNA-23a-5p is a biomarker for gouty arthritis and promotes inflammation in rats of gouty arthritis via MyD88/NF-κB pathway by targeting TLR2.
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Article Info
Published In
Journal
Archives of Rheumatology
Volume / Issue
Volume 37 · Issue 4 · December 2022
Pages
536-546
History
Published Online
December 31, 2022
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Affiliations
1
Fang Li
Department of Rheumatism and Immunology, Hebei General Hospital, Shijiazhuang, China
2
Jian-Hua Yao
Department of Geratology, The Second Hospital of Hebei Medical University, Shijiazhuang, China
3
Li Li
Department Ultrasonograph, Hebei General Hospital, Shijiazhuang, China
4
Qian Nie
Hebei General Hospital, Medicai Examination Center, Shijiazhuang, China
5
Jing-Jing Cao
Department of Rheumatism and Immunology, Hebei General Hospital, Shijiazhuang, China
6
Xiao-Ran Ning
Department of Rheumatism and Immunology, Hebei General Hospital, Shijiazhuang, China
Cite this Article
Fang Li, Jian-Hua Yao, Li Li, Qian Nie, Jing-Jing Cao, & Xiao-Ran Ning. (2022). MiRNA-23a-5p is the biomarkers for gouty arthritis and promotes inflammation in rats of gouty arthritis via MyD88/NF-κB pathway by induction TLR2. Archives of Rheumatology, 37(4), 536–546. https://doi.org/10.46497/ArchRheumatol.2022.9236
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